Methodologies

IHC Methodology For Research
Molecular Testing

IHC Methodology For Research

Immunohistochemistry is the localization of antigens in tissue sections by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold.

Since immunohistochemistry involves specific antigen-antibody reaction, it has advantages over traditionally used special and enzyme staining techniques that identify only a limited number of proteins, enzymes and tissue structures. Therefore, immunohistochemistry has become a crucial technique and widely used in many medical research laboratories as well as clinical diagnostics.

There are numerous immunohistochemistry methods that may be used to localize antigens, therefore, immunohistochemistry performed at PhenoPath Laboratories selects the most suitable method for your research project. This is based on parameters such as the type of specimen under investigation and the degree of sensitivity required.

Molecular Testing

In situ hybridization services performed at PhenoPath Laboratories employ non-radioactive techniques including digoxigenin-, biotin-, or fluorescein-labeled probes, and utilize tyramine-based amplification systems. Screening can be performed on cell lines or deparaffinized, formalin fixed tissues.


In situ hybridization for von Willebrand's factor (vWF) positive on blood vessels	Demonstration of high level amplification of HER2 gene using fluorescence in situ hybridization (FISH)